Effect of TNF autocrine signaling on dosage-dependent NF-kappaB response to lipopolysaccharide stimulation

We will present the dosage-dependent characteristics of NF-kappaB translocation patterns from single macrophages stimulated by E. Coli lipopolysacchride. The NF-kappaB translocation patterns in single cells are found to be quite heterogeneous: The patterns are more heterogeneous with low dosage stimulation than with high dosage stimulation. For low dosage stimulation, most of cells take a rising pattern and we demonstrate that it is due to the TNFalpha autocrine signaling effect. The above results are predicted and explained by a computational model, and corroborated and verified by a single cell fluorescence imaging technique.