Detection of novel cardiovascular disease factor using electrochemical impedance spectroscopy with gold nano-thin-film electrodes

ORAL

Abstract

Cardiovascular disease (CVD) is a leading cause of death worldwide. Angiotensin-converting enzyme 2 (ACE2) is a novel plasma biomarker for CVD. Traditional methods for ACE2 detection, such as quenching fluorescent substrates (QFS) are expensive, time-consuming, and high background. In this study, we have developed a gold nano-thin-film electrode by plasma sputtering technique for electrochemical impedance spectroscopy (EIS). By using Au-NTF-EIS detection system, the ACE2 concentration can be detected from 0.65 fM to 6.5 pM, within 2 mins. Based on its advantages the risk factors of CVDs can be revealed.

*This work was supported in part by the National Science and Technology Council, Taiwan, R.O.C. (Grant numbers: NSTC-113-2112-M-A49-037, MOST 111-2112-M-A49-025; NSTC 112-2112-M-A49-003; 111-2321-B-A49-007; 112-2321-B-A49-005; 111-2927-I-A49-004; 112-2927-I-A49-001; 110-2923-M-009-005-MY3)

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Presenters

  • Cheng-Wei Du

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, Taiwan, ROC

Authors

  • Cheng-Wei Du

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, Taiwan, ROC

  • Chia-Yu Chang

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, Taiwan, ROC
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University
    • National Yang Ming Chiao Tung University

  • Hao-Syun Chou

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu 30068, Taiwan, ROC
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University

  • Yu-Chun Lin

    • National Yang Ming Chiao Tung University

  • Yu-Chaun Liang

    • Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan

  • Chia-Ching Chang

    • National Yang Ming Chiao Tung University
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University