Reusable Surface Amplified Nanobiosensor for the Detection of Airborne Virus

ORAL

Abstract

We reported a reusable surface-amplified nano biosensor for monitoring airborne viruses with a sub-PFU/mL detection limit. In this work, sandwich structures were formed by attaching target virus molecules to antibody-functionalized magnetic particles and alkaline phosphatase (ALP) enzyme. Then, electrochemical markers were generated by ALPs trapped on the Ni patterns by the external magnetic field, resulting in signal amplification. We detected target hemagglutinin (HA) of influenza A (H1N1) virus down to 10 aM concentration using a single sensor chip. Even after repeated sensing measures over the 18 times, the sensor maintained a consistent sensing signal. Moreover, airborne influenza viruses collected from the air could be measured down to sub-PFU/mL level. Considering the reusability and ultrasensitivity of our sensors, it could be a powerful tool for the monitoring of airborne pathogens and help to prevent epidemics by airborne pathogens.

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Presenters

  • Sunwoo Bang

    • Seoul National University

Authors

  • Sunwoo Bang

    • Seoul National University

  • Junghyun Shin

    • Seoul National University
    • Seoul Natl Univ

  • Pan Kee Bae

    • BioNano Health Guard Research Center (H-GUARD)

  • Haneul Yoo

    • Seoul National University
    • Seoul Natl Univ

  • Jeongsu Kim

    • Department of Physics and Astronomy, Seoul National University

  • Yoonji Choi

    • Seoul National University

  • Hyeong Rae Kim

    • Gas Metrology Group, Korea Research Institute of Standards and Science (KRISS)

  • WanSoo Yun

    • Department of Chemistry, Sungkyunkwan University

  • Yong Beom Shin

    • School of Mechanical Engineering, Yonsei University
    • BioNano Health Guard Research Center (H-GUARD)

  • Yong Beom Shin

    • School of Mechanical Engineering, Yonsei University
    • BioNano Health Guard Research Center (H-GUARD)

  • Seunghun Hong

    • Seoul National University
    • Department of Physics and Astronomy, and Institute of Applied Physics

  • WanSoo Yun

    • Department of Chemistry, Sungkyunkwan University