State-of-the-Art Technologies for Optical Imaging of Biological Tissues: Contrast, Resolution, and Throughput

Optical techniques and instrumentation play a significant role in biomedical science and medicine. State-of-the-art biomedical research requires a high level of sophistication in contemporary optical technologies, including fluorescence contrast, advanced microscopy, volumetric imaging. This talk will provide an overview of applications of optical imaging in biological tissues including methods such as fluorescence and nonlinear contrast, super-resolution, and holography and interferometry. These technologies will be presented in the context of overcoming fundamental limitations of optical imaging to enable in vivo visualization of cellular and subcellular features and interactions; surpassing the diffraction-limit using novel fluorophores and computational methods; and pushing the limits of data acquisition speeds for high spatial/temporal imaging throughput.