Organizational principles of active chromatin at transcription condensates
ORAL
Abstract
Many biomolecular condensates associate with specific chromatin loci in the cell nucleus. Transcription condensates are linked to super-enhancer chromatin domains that are characterized by extended accumulations of transcription factor and coactivator binding sites as well as stereotypic epigenetic marks. Bulk assays suggest that these chromatin marks are required to maintain transcription condensates at specific chromatin loci. We use multi-color super-resolution microscopy to investigate the spatial organization of chromatin and transcription condensates. Our results indicate an enrichment of active chromatin at the condensate surface. We discuss implications for the mechanical interaction between condensates and chromatin as well as structure-function relationships of transcription condensates.
*This work was supported in part by NIH grant R21AI159626, a Scialog Award (Research Corporation for Science Advancement), the UIC Honors College, and the College of Liberal Arts and Science at UIC.
–
Presenters
-
Jan Spille
- University of Illinois at Chicago
- University of Illinois Chicago