Anti-SARS-Cov-2 infection drugs and neutralizing antibody screening by palladium nano-thin-film electrode-based biosensor

POSTER

Abstract

Outbreak of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) has infected more than 240 million people and caused nearly five million death. In order to alleviate the symptoms of viral infections and severe illness, drugs/functional peptides to suppressed the virus infection are highly desired. A rapid and sensitive anti-viral infection drugs screening sensing system has been developed by mimicking the SARS-CoV-2 enters the cell model. Four potential leads have been identified, such as perindoprilat and ramiprilat, which can reduce virus infection 67% and 72%, respectively by palladium nano-thin-film electrode-based biosensor (Pd-NTF biosensor) within 21 mins and at low analyte concentration and small volume (0.1 μg/mL and ~1 μL, estimated total analyte consumption < 4 pg). Furthermore, the titer of neutralizing antibody can be analyzed and the effective titer is as high as 19500.

*This study is supported in part by the Ministry of Science and Technology(MOST), Taiwan(ROC), MOST 110-2923-M-009-005-MY3, M0ST 110-2112-M-A49-005, MOST 109-2327-B-009-001. This work is also supported by the Ministry of Education through the SPROUT Project.

Publication: 1. Kiew L.V., et al., (2021) Biosencers&Bioelectronics 183,11321
2. Chang C.-Y., et al., (2019) Appl. Phys. Lett.114,093702

Presenters

  • Yi-Xuan Huang

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan

Authors

  • Yi-Xuan Huang

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan

  • Lik-Voon Kiew

    • Department of pharmacology,Faculty of Medicine,University of Malaya, 50603 Kuala Lumpur,Malaysia

  • Chia-Hsing Cheng

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan

  • Chia-Yu Chang

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan

  • Yen chen Chen

    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan

  • Chia-Ching Chang

    • Department of Biological Science and Technology, Natl Chiao Tung Univ
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Taiwan
    • Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan