Patterned shear flows drive folding during organogenesis

ORAL

Abstract

In morphogenesis, living matter translates information encoded at the cellular scale into complex shape transformations. How does in-plane tissue patterning generate out-of-plane deformation needed to sculpt complex forms? A dramatic example of this theme is the fruit fly midgut -- a tube that folds and coils into a helical configuration in only two hours. Here, using a combination of light-sheet microscopy, genetics, computer vision, and tissue cartography, we reconstruct the full 3D shape dynamics and identify the driving sources of the shape change by linking out-of-plane motion to the cellular flow and active contraction patterns. Optogenetic manipulation of contractility in the endoderm and muscle layers decouples the role of each in sculpting the bilayer organ, enabling a simple mechanical model for midgut morphogenesis.

*Helen Hay Whitney Foundation

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Presenters

  • Noah Mitchell

    • University of California, Santa Barbara
    • James Franck Institute, University of Chicago

Authors

  • Noah Mitchell

    • University of California, Santa Barbara
    • James Franck Institute, University of Chicago

  • Dillon Cislo

    • University of California, Santa Barbara

  • Suraj Shankar

    • Harvard University
    • Physics, Harvard University

  • Zvonimir Dogic

    • Physics, University of California, Santa Barbara
    • University of California, Santa Barbara
    • University of California at Santa Barbara, Santa Barbara
    • University of California, Santa Barbara, Harvard University, Brandeis University

  • Boris I Shraiman

    • University of California, Santa Barbara
    • KITP, University of California, Santa Barbara

  • Sebastian Streichan

    • University of California, Santa Barbara