Single polypeptide antibody functionalized electrochemical probe development for enterovirus detection

ORAL

Abstract

Enterovirus 71 affects global public health and causes the millions of infections in human via oral and respiratory infection every year. Conventional detection approaches are through cell culture and PCR assay processes. However, these processes are time consuming. Therefore, a rapid, sensitive detection of enterovirus is required. Electrochemical impedance spectroscopy (EIS) is widely used for antigen-antibody interaction to measure the impedance change of the bio-sensing probe. However, conventional antibody is not stable for EIS probe. We have developed a single polypeptide antibody for enterovirus 71 detection. This single polypeptide antibody can conjugate with EIS electrode directly via self-assembly process. Moreover, this functionalized sensing probe can bind with EV71 sensitively and selectively within a few minutes by EIS. This single polypeptide antibody is relatively stable to detect enterovirus and may reduce the risk of enterovirus outbreak.

*This project was supported by MOST grant MOST 107-2112-M-009-016-MY3. This work is particularly supported by the Ministry of Education through the SPROUT Project-Center for Intelligent Drug Systems and Smart Biodevices (IDS2B) of NCTU, Taiwan.

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Presenters

  • Yi-Xiang Lu

    • Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan

Authors

  • Yi-Xiang Lu

    • Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan

  • Chia-Yu Chang

    • Natl Chiao Tung Univ
    • Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan

  • Wen-Bin Fan

    • Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan

  • Jyh-Yuan Yang

    • Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan

  • Chia-Ching Chang

    • Natl Chiao Tung Univ
    • Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan
    • Department of Biological Science and Technology, National Chiao Tung University, Taiwan