Catalytic enzymes are active matter

ORAL

Abstract

Recent studies suggest that the enhanced diffusion of catalytically-active enzymes is promoted by super-diffusive “kicks” generated by the catalytic events. Pursuing this idea, we have explored methods to vary systematically the turnover frequency, kcat, which defines how many substrate molecules are consumed in a given time. Our measurements using fluorescence correlation spectroscopy in super-resolution mode (FCS-STED) enable us to illuminate this matter. The contrast between motions in the presence of inhibitor and of substrate is especially interesting.

–

Presenters

  • Ah-Young Jee

    • Institute for basic science

Authors

  • Ah-Young Jee

    • Institute for basic science

  • Tsvi Tlusty

    • Institute for basic science
    • Center for Soft and Living Matter, Institute of Basic Science
    • Center for Soft and Living Matter, Institute for basic science

  • Steve Granick

    • Institute for basic science
    • Center for soft and living matter, Institute for Basic Sciences
    • Institute for Basic Science
    • Center of Soft and Living Matter, Institute for Basic Science
    • Ulsan National Institute of Science and Technology
    • Center for Soft and Living Matter, Korea Institute of Basic Science
    • IBS Center for Soft and Living Matter