Novel strategies for holographic optical activation of neurons

ORAL

Abstract

In-vivo two photon imaging of neuronal activity in the higher sensory cortical layers has revealed that the spatial arrangements of neurons involved in processing sensory stimuli is complex. Recent advances in optogenetics have allowed for optical manipulation of neurons, and holographic microscopy has enabled us to selectively target neurons for activation. Here we introduce the use of extended holographic patterns with high planar selectivity for photo activation of neurons. Our approach takes advantage of a lens function to reduce off-target speckles. We find that in addition to reducing speckles, our approach also generates target patterns with a higher degree of uniformity. We demonstrate the feasibility of this method for in vivo studies of the auditory cortex.

*Supported by BRAIN initiative grant U01NS090569 (PK, WL) and University of Maryland Seed Support.

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Presenters

  • Samira Aghayee

    • University of Maryland, College Park
    • Institute of Physical Sciences, University of Maryland, College Park
    • Biophysics, University of Maryland, College Park

Authors

  • Samira Aghayee

    • University of Maryland, College Park
    • Institute of Physical Sciences, University of Maryland, College Park
    • Biophysics, University of Maryland, College Park

  • Patrick Kanold

    • University of Maryland, College Park

  • Wolfgang Losert

    • University of Maryland, College Park
    • Department of Physics, University of Maryland
    • Institute of Physical Sciences, University of Maryland, College Park
    • Department of Physics, University of Maryland,College Park
    • Physics, University of Maryland, College Park
    • Department of Physics, University of Maryland-College Park
    • IREAP, IPST, University of Maryland, College Park
    • Departments of Physics, IPST and IREAP, University of Maryland, College Park