Measurement of In vitro Cancer Tumor Hypoxia

ORAL

Abstract

Tumors are characterized as swamps: abnormal and disordered tissue masses with highly stressful conditions of hypoxia, low pH, low nutrient conditions due to a combination of rapid cell growth, lack of vasculature and altered metabolism. While for normal cells that combination would be lethal, for cancer cells it provides a genotoxic environment they are adapted to. We show here using a phosphorescence lifetime imaging (PLIM) technology based oxygen sensor to monitor the local O$_{2}$ level in a extended two dimensional array of cancer cells with strong and mixed gradients to nutrients and O$_{2}$ using a novel pure diffusional three dimensional microfabricated technology the emergence of highly hypotoxic dormant cell metapopulations.

*This work was supported by NSF PHY-1659940.

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Presenters

  • Robert Austin

    • Princeton University

Authors

  • Yihiua Zhao

    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518

  • Robert Austin

    • Princeton University

  • Ke-Chih Lin

    • Princeton University

  • James Sturm

    • Princeton University

  • Junli Qu

    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518