Anti-cancer drug containing apolioprotein B lipoparticle reconstitution and tracking in living cell

ORAL

Abstract

Radicicol (Rad), an Hsp 90 inhibitor, is an antitumor drug which loses its antitumor activity during delivery process, in vivo. By adding Rad in the reconstituting apolioprotein B (apoB) process, a Rad containing reconstituted apoB lipoparticle (rABL) complex (Rad@rABL) can be reconstituted and characterized by photonic spectroscopies, differential scanning calorimetry and super resolution imaging. Interestingly, the Rad@rABL single molecule can be tracked within the living cell. The drug delivery route to the mitochondria in the Hep G2 cell line can be monitored. Moreover, the anticancer efficacy of Rad can be enhanced. In summary, rABL is an excellent and native self-assembly drugs carrier and living cell tracking agent.

*This study is supported in part by the Ministry of Science and Technology (MOST), Taiwan (ROC) MOST 107-2112-M-009-016-MY3

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Presenters

  • Wei-Ping Chang

    • Department of Biological Science and Technology, National Chiao Tung University,Taiwan

Authors

  • Wei-Ping Chang

    • Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Chung Ching Lin

    • Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Hsueh-Liang Chu

    • Department of Biological Science and Technology, National Chiao Tung University,Taiwan

  • Po-Yen Lin

    • Academia Sinica, Taiwan
    • Division of Core Facilities Imaging, Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan

  • Yu-Chuan Liang

    • Agricultural Biotechnology Research Center, Academia Sinica, Taiwan.

  • Caleb G. Chen

    • Department of Hematology-Oncology, Mackay Memorial Hospital, Taiwan

  • Chia-Ching Chang

    • Biological Science and Technology, National Chiao Tung University
    • Department of Biological Science and Technology, National Chiao Tung University,Taiwan
    • Department of Biological Science and Technology, National Chiao Tung University，Taiwan