Out-of-plane beating components of active axonemes isolated from Chlamydomonas reinhardtii

ORAL

Abstract

Cilia and flagella are ubiquitous in the living world. They are essential for micro-scale driven transport of fluids or cells by cilia/flagellar beating. Their slender bodies are composed of a microtubule/molecular motor structure that when taken independently are called an axoneme. Axonemes move by bending waves that emerge from the interplay between internal stresses generated by dynein motor proteins . Here we use the novel multi-plane phase contrast imaging technique to record the three dimensional beating pattern of isolated axonemes from Chlamydomonas reinhardtii that beat in the vicinity of a substrate. We measure the torsion of the axoneme along the contour length with high spatiotemporal resolution. High precision information on out-of-plane beating component of axonemes allows us to check the validity of the resistive-force theory.

*We acknowedge MaxSyBio consorsium, which is jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society.

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Presenters

  • Azam Gholami

    • Max-Planck-Gesellschaft
    • Max-Planck Institute for Dynamics and Self-organization, Göttingen, Germany

Authors

  • Azam Gholami

    • Max-Planck-Gesellschaft
    • Max-Planck Institute for Dynamics and Self-organization, Göttingen, Germany

  • Soheil Mojiri

    • Göttingen University

  • Albert Johann Bae

    • Department of Biomedical Engineering, University of Rochester

  • Jörg Enderlein

    • Göttingen University
    • 3rd Institute of Physics - Biophysics, University of Göttingen
    • Third Physical Institute, Georg-August-University Goettingen

  • Eberhard Bodenschatz

    • Fluid Physics, Pattern Formation, and Biocomplexity, Max-Planck-Institute for Dynamics and Self-Organization
    • Max Planck Institute for Dynamics and Self-Organization
    • Max-Planck Institute for Dynamics and Self-organization, Göttingen, Germany
    • Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany