X-ray Photon Correlation Spectroscopy Measurements of Protein Diffusion in Concentrated Alpha Crystallin Suspensions

ORAL

Abstract

The dynamics of concentrated suspensions of the crystallin eye-lens protein alpha crystallin was measured using x-ray photon correlation spectroscopy. For pure alpha crystallin suspensions the diffusive dynamics near the peak in the hard sphere structure are well described by a polydisperse hard sphere model. The intermediate scattering factor can be described by a stretched exponential function, and the concentration dependence of the relaxation time agrees with predictions from Langevin dynamics simulations.

*Research was supported by the National Eye Institute of the National Institutes of Health under Award Number R15EY018249. Work at the APS was supported by DOE Contract No. DE-AC02-06CH11357. UFXC32k detector development was supported by the national center for Research and Development, Poland PBS1/A3/12/2012

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Presenters

  • Laurence Lurio

    • Physics, Northern Illinois University

Authors

  • Laurence Lurio

    • Physics, Northern Illinois University

  • Preeti Vodnala

    • Physics, Northern Illinois University

  • Vidanage Nuwan C Karunaratne

    • Northern Illinois University

  • George Thurston

    • School of Physics and Astronomy, Rochester Institute of Technology
    • Rochester Institute of Technology
    • Physics, Rochester Institute of Technology

  • Michael Vega

    • Chemistry and Biochemistry, Northern Illinois University

  • Elizabeth Gaillard

    • Chemistry and Biochemistry, Northern Illinois University

  • Suresh Narayanan

    • Argonne
    • Argonne National Laboratory

  • Alec Russell Sandy

    • Argonne National Laboratory

  • Qingteng Zhang

    • Argonne National Laboratory

  • Eric Dufresne

    • Argonne National Laboratory

  • Giuseppe Foffi

    • Laboratoire de Physique des Solides,, Universite Paris-Saclay

  • Pawel Grybos

    • AGH University of Science and Technology,

  • Piotr Kmon

    • AGH University of Science and Technology,

  • Piotr Maj

    • AGH University of Science and Technology,

  • Robert Szczygiel

    • AGH University of Science and Technology,