Measuring and Simulating Cellular Flows during Spindle Positioning

ORAL

Abstract


A cell is a complex fluidic environment in which many fundamental biological processes take place. One such is the proper positioning and elongation of the mitotic spindle which is crucial for chromosome segregation and asymmetric cell division, and involves the interaction of microtubule assemblies with motor-proteins and subcellular organelles. In a combined experimental and computational study, we use cytoplasmic flow measurements and computational fluid dynamics to argue that proper positioning is primarily achieved by the action of motor-proteins bound to the cell boundary.

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Presenters

  • Ehssan Nazockdast

    • Applied Physical Sciences, University of North Carolina
    • New York Univ NYU

Authors

  • Ehssan Nazockdast

    • Applied Physical Sciences, University of North Carolina
    • New York Univ NYU

  • Hai Yin Wu

    • School of Engineering, Harvard University

  • Daniel Needleman

    • School of Engineering, Harvard University
    • SEAS, Harvard University
    • MCB/SEAS, Harvard University

  • Michael Shelley

    • Center for Computational Biology, Simons Foundation; Courant Institute of Mathematical Science, New York University
    • New York University
    • Courant Institute/Flatiron Institute
    • Center for Computational Biology, Flatiron Institute, Simons Foundation
    • Flatiron Institute, Simons Foundation
    • Center for Computational Biology, Flatiron Institute
    • Flatiron Institute
    • CCB, Flatiron Institute