Lattice Light Sheet Microscopy of Endocytosis in Macrophages
ORAL
Abstract
Live cell, volumetric fluorescence imaging using a Lattice Light Sheet microscope, developed in collaboration with HHMI Janelia Farm Research Campus[1] is used to visualize endocytosis of B cells by macrophages derived from mouse bone marrow. Cells are gene edited to express fluorescence of specific organelles and/or labeled with fluorescent antibodies. Volumetric, multi-color fluorescence imaging reveals cellular level dynamical details of this process. Discussion of major findings, instrumentation, and data visualization will be presented[2].
*[1]The Bessel Beam referenced in this research was used under license from Howard Hughes Medical Institute, Janelia Farm Research Campus.
[2]This material is based upon work supported by the National Science Foundation/EPSCoR Cooperative Agreement #IIA-1355423. Any opinions, findings, and conclusio
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Presenters
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Robert Anderson
- Nanoscience and Nanoengineering, South Dakota Sch Mines & Tech