Impedimetric Zika and Dengue Biosensor based on Functionalized Graphene Oxide Wrapped Silica Particles

ORAL

Abstract

A composite of 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped on SiO2 particles (SiO2@APTES-GO) was prepared via self-assembly. Transmission electron microscopy (TEM) and ATR-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO2 particles by the APTES-GO sheets. An impedimetric biosensor was constructed and used to sensitively detect Zika and dengue DNA and RNA via primer hybridization using different oligonucleotide sequences. The results demonstrate that the SiO2@APTES-GO electrode materials provide enhanced RNA detection sensitivity with selectivity and detection limit (1 femto-Molar), compared to both APTES-GO and APTES-SiO2. The three-dimensional structure, higher contact area, electrical properties and the ability for rapid hybridization offered by the SiO2@APTES-GO resulted in a successful design of a Zika and dengue biosensor with the lowest detection limit reported to date. We are in the process of developing a platform for multiple viral detection for point-of-care diagnostics for arthropode borne viral infectious diseases.

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Authors

  • Seon-Ah Jin

    • Perdue University
    • Schools of Materials and Electrical and Computer Engineering, Purdue University, USA.
    • School of Materials Engineering, Purdue University, USA.
    • School of Materials Engineering, Purdue University, USA

  • Seon-Ah Jin

    • Perdue University
    • Schools of Materials and Electrical and Computer Engineering, Purdue University, USA.
    • School of Materials Engineering, Purdue University, USA.
    • School of Materials Engineering, Purdue University, USA

  • Lia A. Stanciu Stanciu

    • School of Materials Engineering, Purdue University, USA

  • Shishir Poudyal

    • Department of Biological Sciences, Purdue University, USA

  • Richard J. Kuhn

    • Department of Biological Sciences, Purdue University, USA