Analysis of T lymphocyte activation measured by Super-Resolution Microscopy

ORAL

Abstract

Tight regulatory control of the activation signal in T lymphocytes is necessary to prevent the immune response from getting too large or persisting too long. Utilizing cutting-edge super-resolution imaging technologies in combination with quantitative image analysis, we explore one aspect of this regulation in activated cells: the dynamics of the protein MALT1. Our goal is to analyze how the motion of MALT1 within the cell affects the transduction and regulation of the activation signal. A focus of our analysis is to measure anisotropies in the spatial organization of MALT1 and the shape of the related larger scale protein complex that it is a part of, the POLKADOTS Signalosome.

–

Authors

  • Leonard Campanello

    • University of Maryland at College Park

  • Maria Traver

    • Uniformed Services University of the Health Sciences

  • Hari Schroff

    • National Institute of Biomedical Imaging and Bioengineering

  • Brian Schaefer

    • Uniformed Services University of the Health Sciences

  • Wolfgang Losert

    • University of Maryland College Park
    • Univ of Maryland-College Park
    • University of Maryland at College Park