How to Concentrate Genomic Length DNA in a Microfabricated Array

ORAL

Abstract

We demonstrate that a microfabricated bump array can concentrate genomic-length DNA molecules efficiently at continuous, high flow velocities, up to 40 ?m/s, if the single-molecule DNA globule has a sufficiently large shear modulus.. Increase in the shear modulus is accomplished by compacting the DNA molecules to minimal coil-size using polyethylene glycol (PEG) derived depletion forces. We map out the sweet spot where concentration occurs as a function of PEG con- centration, flow speed, and bump array parameters using a combination of theoretical analysis and experiment. Purification of DNA from enzymatic reactions for next-generation DNA-sequencing libraries will be an important application of this development.

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Authors

  • Yu Chen

    • Princeton University

  • Ezra Abrams

    • Sage Science Inc.

  • Christian Boles

    • Sage Science Inc.

  • Jonas Pedersen

    • Danish Technical University

  • Henrik Flyvbjerg

    • Danish Technical University

  • James Sturm

    • Princeton University

  • Robert Austin

    • Princeton University
    • Princeton Unversity