Metallic Glass Wire Based Localization of Kinesin/Microtubule Bio-molecular Motility System

ORAL

Abstract

We report electrophoretic accumulation of microtubules along metallic glass (Pd$_{42.5}$Cu$_{30}$Ni$_{7.5}$P$_{20}$) wires free-standing in solution. Microtubules are dynamic cytoskeletal filaments. Kinesin is a cytoskeletal motor protein. Functions of these bio-molecules are central to various dynamic cellular processes. Functional artificial organization of bio-molecules is a prerequisite for transferring their native functions into device applications. Fluorescence microscopy at the individual-microtubule level reveals microtubules aligning along the wire axis during the electrophoretic migration. Casein-treated electrodes are effective for releasing trapped microtubules upon removal of the external field. Furthermore, we demonstrate gliding motion of microtubules on kinesin-treated metallic glass wires. The reversible manner in the local adsorption of microtubules, the flexibility of wire electrodes, and the compatibility between the wire electrode and the bio-molecules are beneficial for spatio-temporal manipulation of the motility machinery in 3 dimensions.

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Authors

  • K. Kim

    • WPI-AIMR, Tohoku University, Japan

  • A. Sikora

    • WPI-AIMR, Tohoku University, Japan

  • S. Yaginuma

    • WPI-AIMR, Tohoku University, Japan

  • K.S. Nakayama

    • WPI-AIMR, Tohoku University, Japan

  • H. Nakazawa

    • Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Japan

  • M. Umetsu

    • WPI-AIMR/Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Japan

  • W. Hwang

    • Biomedical Engineering/Materials Science and Engineering, Texas A\&M University; School of Computational Sciences, KIAS, Korea

  • W. Teizer

    • WPI-AIMR, Tohoku University, Japan; Physics and Astronomy/Materials Science and Engineering, Texas A\&M University