High-speed Membrane Imaging with Digital Holography

ORAL

Abstract

Lipid membranes can change on timescales faster than traditional three dimensional imaging tools can follow. Digital holography offers a the potential to observe observe membranes in 3D at 1000 Hz or greater to resolve dynamics down to thermal fluctuations. This works because holography encodes 3D information into a single 2D image, allowing imaging limited only by camera speed. However, precise quantitative interpretation of holograms has proved challenging for samples of any complexity. To address this limitation, I am developing methods based on the discrete dipole approximation and a new mathematical approach to solving inverse problems. I will present these methods and preliminary measurements of membrane dynamics using holography.

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Authors

  • Thomas G. Dimiduk

    • Harvard
    • Harvard University, Dept. of Physics

  • Amy Chen

    • Harvard

  • Laura Arriaga

    • Harvard

  • Vinothan N. Manoharan

    • School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA
    • Harvard SEAS and Physics
    • Harvard University
    • Physics Department and SEAS Harvard University
    • Harvard University, School of Engineering and Applied Sciences and the Department of Physics
    • Harvard
    • Harvard University, School of Engineering and Applied Sciences and Dept. of Physics