Ligation of Complementary Oligomers in Liquid Crystals of nanoDNA

ORAL

Abstract

The chromonic stacking mode of short oligomeric DNA upon forming liquid crystalline phases presents an intriguing possibility for liquid crystal autocatalysis, the promotion, by LC ordering, of chemical synthesis that stabilizes LC ordering. In such a scenario the concentration and physical organization of ligation reactants and the fluidity of the liquid crystal phase promotes the appropriate chemical ligation of oligomers. Because it is a mode of elongation free of other catalysts, this offers a tantalizing means of oligonucleotide self-elongation that might have implications in prebiotic life. We present here work toward elucidating possible catalytic enhancement by liquid crystalline phase formation. Ligation approaches based on water soluble carbodiimide base activation and photopolymerization will be presented.

*This work was supported by NSF MRSEC Grant DMR-0820579.

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Authors

  • Gregory Smith

    • LCMRC, CU Boulder
    • Liquid Crystal Materials Research Center, University of Colorado, Boulder (CO) U.S.A.

  • David Walba

    • LCMRC, CU Boulder

  • Noel Clark

    • University of Colorado at Boulder
    • LCMRC, University of Colorado, Boulder
    • LCMRC, CU Boulder
    • Dept. of Physics, University of Colorado
    • Liquid Crystal Materials Research Center, University of Colorado at Boulder
    • Physics, University of Colorado at Boulder
    • Department of Physics and Liquid Crystal Material Research Center, University of Colorado-Boulder
    • Liquid Crystal Materials Research Center, University of Colorado, Boulder (CO) U.S.A.

  • Weixian Xi

    • Chemical Engineering, CU Boulder

  • Tao Gong

    • Chemical Engineering, CU Boulder

  • Christopher Bowman

    • Chemical Engineering, CU Boulder

  • Tommaso Fraccia

    • University of Milan

  • Giuliano Zanchetta

    • University of Milan

  • Tommaso Bellini

    • University of Milan