Interactions between cyclic cell penetrating peptides and lipid membranes

ORAL

Abstract

Cyclic peptides exhibit strong enhancement in receptor-binding affinity, specificity, and stability relative to their linear counterparts, partially due to their reduced conformational freedom. In this work, we examine cyclic versions of cell penetrating peptides. Using small-angle x-ray scattering (SAXS) measurements, we show that cyclic polyarginine peptides generate saddle-splay curvature more efficiently than their linear counterparts, We show how this increase in induced saddle splay curvature impinges on the efficiency of cell penetration in a series of giant vesicle and intracellular trafficking experiments.

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Authors

  • Kun Zhao

    • Bioengineering Department, University of California, Los Angeles

  • Tao Liu

    • Chemistry Department, the Ohio State University

  • Mike Choe

    • Bioengineering Department, University of California, Los Angeles

  • Daniel Kamei

    • Bioengineering Department, University of California, Los Angeles

  • Dehua Pei

    • Chemistry Department, the Ohio State University

  • Gerard Wong

    • University of California, Los Angeles, Bioengineering Department
    • University of California Los Angeles
    • Bioengineering Department, University of California, Los Angeles
    • Bioengineering Dept., University of California Los Angeles, Dept. of Physics University of Illinois Urbana Champaign