Interaction between Cell Penetrating pVEC and cell membranes

ORAL

Abstract

Vascular Endothelial Cadherin (VEC) is a transmembrane-spanning glycoprotein that belongs to the family of cell adhesion molecules and plays an active role in control of vascular permeability and angiogenesis. PVEC, an 18 amino acid domain, has been shown to be able to traverse cell membranes with attached macromolecules. pVEC is an amphiphilic molecule with a high content of basic amino acids resulting in a net positive charge. Electrostatic and hydrophobic interactions can perturb membrane self-assembly and stability and are likely to be responsible for peptide uptake. We use synchrotron x-ray scattering and confocal microscopy to examine the phase behavior of the pVEC lipid system, and its relation to membrane permeation mechanisms.

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Authors

  • Abhijit Mishra

    • Bioengineering Dept., University of California Los Angeles

  • Ghee Hwee Lai

    • Bioengineering Dept., University of California Los Angeles, Dept. of Physics University of Illinois Urbana Champaign

  • Nathan Schmidt

    • Bioengineering Dept., University of California Los Angeles, Dept. of Physics University of Illinois Urbana Champaign

  • Gerard Wong

    • University of California, Los Angeles, Bioengineering Department
    • University of California Los Angeles
    • Bioengineering Department, University of California, Los Angeles
    • Bioengineering Dept., University of California Los Angeles, Dept. of Physics University of Illinois Urbana Champaign