Single-Molecule Tracking of Polymers on Surfaces

Juan Guan; Bo Wang; Stephen Anthony; Sung Chul Bae; Subhalakshmi Kumar; Steve Granick

Single-Molecule Tracking of Polymers on Surfaces

ORAL

Abstract

Single-molecule tracking technique is, for the first time, applied to study adsorbed polymers on surfaces. Fluorescently-labeled poly (ethylene glycol) (PEG) chains are allowed to freely adsorb from dilute aqueous solutions to various surfaces with different affinity. After rinsing away any non-adsorbed chains, the motion of the surface-bound species is tracked using objective-based total internal reflection fluorescence microscopy (TIRFM). Using our single particle tracking algorithm, individual chain behaviors are analyzed with sub-diffraction resolution.

March 18, 2010, 1:03 PM – March 18, 2010, 1:15 PM

Authors

Juan Guan
- U of Illinois Urbana-Champaign
Bo Wang
- U of Illinois Urbana-Champaign
- Department of Materials Science and Engineering, University of Illinois
Stephen Anthony
- University of Illinois
- U of Illinois Urbana-Champaign
Sung Chul Bae
- U of Illinois Urbana-Champaign
- Department of Materials Science and Engineering, University of Illinois
Subhalakshmi Kumar
- U of Illinois Urbana-Champaign
Steve Granick
- University of Illinois
- Department of Materials Science and Engineering, University of Illinois
- University of Illinois, Department of Materials Science and Engineering
- U of Illinois Urbana-Champaign
- University of Illinois at Urbana Champaign
- Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign
- University of Illinois, Urbana Champaign