Ice-binding protein investigation using microfluidic devices

ORAL

Abstract

Ice-binding proteins (IBPs) inhibit ice crystal growth and recrystallization. We have developed a novel microfluidic device capable of precise local temperature control in order to grow single ice crystals. This device allows us to expose an ice crystal to an adjustable IBP concentration. We have used this device in conjunction with fluorescence microscopy to examine the affinities hyperactive IBPs have to specific ice planes in comparison with those of a moderately active fish IBP. We also demonstrate that hyperactive IBPs bind irreversibly to ice surfaces. The direct visualization of IBPs on ice using the microfluidic devices reveal the kinetics of attachment of these proteins to ice surfaces, as well as their concentration effects and facet preferences.

*Funded by CIHR, BNNT and NQPI at Ohio University, and Sigma Xi Grant-in-Aid of Research.

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Authors

  • Yeliz Celik

    • Ohio University, Athens, OH

  • Natalya Pertaya

    • Ohio University, Athens, OH

  • Christophere P. Garnham

    • Queens University, Kingston, ON, Canada

  • Peter L. Davies

    • Queens University, Kingston, ON, Canada

  • Ido Braslavsky

    • Ohio University, Athens, OH