Magnetic detection of biotin-streptavidin binding using InAs quantum well $\mu $-Hall sensor

ORAL

Abstract

Magnetic sensors are a key component in any high-sensitivity, rapid-response, and portable platform for magnetic biosensing. InAs quantum well micro-Hall sensors have shown high potential for such a role due to their low noise level and capability to detect single micron- sized or smaller superparamagnetic beads suitable for biosensing$^{1}$. Here we present successful selective biotinylation of InAs micro-Hall sensors and directed self-assembly of 350 nm streptavidin-coated superparamagnetic beads via the biotin-streptavidin interaction. Two Hall crosses with three and two beads produced detection signals with S/N ratio of 21.3 dB and 18.4 dB respectively. In addition, our progress for \textit{in situ} detection of micron-sized magnetic beads using microfluidic channel will be presented. $^{1}$G. Mihajlovic et al., APL 87, 112502 (2005) This work was supported by NIH NIGMS GM079592.

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Authors

  • Khaled Aledealat

    • Department of Physics and MARTECH, Florida State University

  • K. Chen

    • Department of Physics and MARTECH, Florida State University

  • G. Mihajlovic

    • Materials Science Division, Argonne National Laboratory

  • P. Xiong

    • Department of Physics, MARTECH and INSI, Florida State University

  • G. Strouse

    • Department of Chemistry and Biochemistry and INSI, Florida State University

  • P.B. Chase

    • Department of Biological Science and INSI, Florida State University

  • S. von Moln\'ar

    • Department of Physics, MARTECH and INSI, Florida State University

  • M. Field

    • Teledyne Scientific Company LLC

  • G.J. Sullivan

    • Teledyne Scientific Company LLC