Extraction of complementary from non-complementary DNA sequences through phase separation and centrifugation
ORAL
Abstract
Double stranded deoxyribonucleic acid (DNA) is known to form lyotropic liquid crystal (LC) phases, nematic and then columnar with increasing DNA concentration in water. Single stranded (DNA) does not form liquid crystal phases. We study the phase separation of both long (900bp) and short (6-20bp) DNA. In the mixture solution of a self complementary sequences (scDNA) and non complementary sequences (nscDNA), the scDNA forms DNA double helices and hence forms LC phases while the nscDNA stays in the isotropic phase, the LC appearing in the form of phase separated droplets. We report results of the use of centrifugation to produce complete spatial segregation of complementary and noncomplementary DNA, based on their different LC-formation tendencies.
*This work is supported by NSF MRSEC Grant DMR 0213918 and NSF Grant ITS-0606528.
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Authors
Taiquitha Robins
Jackson State University, Jackson, MS
Dacia McPherson
Department of Physics, University of Colorado, Boulder, CO
Chenhui Zhu
Department of Physics, University of Colorado, Boulder, CO
Mark Moran
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO
Dave Walba
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO
Giuliano Zanchetta
Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universit\`a di Milano, Milano, Italy
Tommaso Bellini
Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universit\`a di Milano, Milano, Italy
Noel Clark
Department of Physics and Liquid Crystal Material Research Center, University of Colorado at Boulder
Department of Physics, The University of Colorado, Boulder, CO 80309
Department of Physics and Liquid Crystal Materials Research Center, University of Colorado, Boulder, CO
