Single liposome tracking in dense suspensions of stabilized liposomes

ORAL

Abstract

Methods developed to stabilize phospholipid vesicles against fusion, up to volume fraction around 80{\%}, enable one to perform single-particle tracking on these soft, flexible, hollow objects. Stabilization is accomplished by studding the outer leaflet with charged nm-sized particles. Image analysis of time trajectories, obtained using epifluorescence imaging, was performed at sub-pixel resolution. This talk will emphasize aspects of curiously heterogeneous dynamics and also quantification of ``cage'' size in this system. Taken together, this system of charged, polydisperse, flexible objects displays rich dynamics that contrasts acutely with known behavior for hard-sphere dense particle systems.

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Authors

  • Yan Yu

    • Dept of Materials Science and Engineering, Univ of Illinois at Urbana-Champaign

  • Stephen Anthony

    • Department of Chemistry, University of Illinois
    • Dept of Chemistry, Univ of Illinois at Urbana-Champaign
    • Dept. of Chemistry, University of Illinois at Urbana and Champaign

  • Liangfang Zhang

    • Dept of Chemical Engineering, Univ of Illinois at Urbana-Champaign

  • Angelo Cacciuto

    • Department of Materials Science and Engineering and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign
    • University of Illinois at Urbana-Champaign
    • Dept of Materials Science and Engineering, Univ of Illinois at Urbana-Champaign

  • Steve Granick

    • Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign
    • University of Illinois at Urbana-Champaign
    • University of Illinois
    • Dept of Materials Science and Engineering, Univ of Illinois at Urbana-Champaign
    • Dept. of Material Science and Engineering, University of Illinois at Urbana and Champaign