Lipid Coupling in Asymmetric Supported Lipid Bilayers Revealed by Fluorescence Correlation Spectroscopy

ORAL

Abstract

In biological systems, phospholipids asymmetry in two leaflets is a key feature of cell membranes for membrane biogenesis, intracellular fusion and signal transduction. Detailed information of the interactions and dynamics of the asymmetric membranes is paramount for design of applications. Here we use fluorescence correlation spectroscopy (FCS) to measure the coupling between 1, 2-dilauroyl-\textit{sn}-glycero-3-phosphocholine (DLPC) and 1, 2-dipalmitoyl-\textit{sn}-glycero-3-phosphocholine (DPPC) in asymmetric planar-supported bilayers (PSLBs), at temperatures where DLPC is in the fluid phase but DPPC is in the gel phase. Asymmetric PSLBs were prepared by placing dilute fluorescent-labeled 1, 2-dimeristoyl-\textit{sn}-glycero-3-phosphoethanolamine (DMPE) in DLPC leaflet as the probe for measuring lateral diffusion within the host leaflet environment. By constructing asymmetric bilayers where DLPC is alternatively in the top and in the bottom leaflet, we compare lipid coupling between the two leaflets with frictional interaction between the leaflets and the nanometer-thick water layer that separates the bottom leaflet from the solid support.

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Authors

  • Yan Yu

    • Dept. of Materials Science \& Engineering, UIUC

  • Liangfang Zhang

    • Dept. of Chemical \& Biomolecular Engineering, UIUC
    • Department of Chemical \& Biomolecular Engineering, UIUC

  • Steve Granick

    • Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign
    • Departments of Physics, Chemistry, and Materials Science and Engineering, University of Illinois at Urbana-Champaign
    • University of Illinois at Urbana-Champaign
    • Dept. of Materials Science \& Engineering, of Physics, of Chemistry, and of Chemical \& Biomolecular Engineering, UIUC
    • Departments of Materials Science and Engineering, of Chemistry, of Physcis, University of Illinois
    • Department of Materials Science and Engineering, Chemistry, Physics, Chemical Engineering, University of Illinois at Urbana-Champaign
    • UIUC, Materials Science and Engineering Department
    • Department of Materials Science \& Engineering, of Chemistry, of Physics, and of Chemical \& Biomolecular Engineering, UIUC
    • Departments of Chemistry and of Material Science, University of Illinois