Dual Syringe Electrospinning of FNfds-modified Hyaluronic Acid

ORAL

Abstract

We described the fabrication of a unique HA nanofibrous scaffold using dual syringe reactive electrospinning. 3$^{'}$-dithiobis(propanoic dihydrazide)-modified HA (HA-DTPH) and Poly (ethylene glycol)-diacrylate (PEGDA) were selected as the cross-linking system. PEO was blended with HA-DTPH to facilitate the fiber formation. Fibronectin functional domains (FNfds) were incorporated with PEGDA and covalently linked to HA via conjugate addition to improve the cell attachment. The as-spun scaffold was soaked into DI water to remove PEO and yield an FNfds-modified HA-DTPH nanofibrous scaffold. Human dermal fibroblasts CF31 were seeded on FNfds-modified HA-DTPH scaffolds. The CF31 fibroblasts showed a unique extended dendritic morphology which is opposed to the typical flattened morphology of cells on regular 2D geometries. Supported by NSF-MRSEC.

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Authors

  • Yuan Ji

    • Dept. of Materials Science, SUNY at Stony Brook

  • Kaustabh Ghosh

    • Dept. of Biomedical Engr., SUNY at Stony Brook

  • Xiaozheng Shu

    • Dept. of Medicinal Chemistry, the University of Utah

  • Jonathan Sokolov

    • Dept. of Materials Science, SUNY at Stony Brook
    • Dept. Mat. Sci. \& Eng., SUNY at Stony Brook

  • Glenn Prestwich

    • Dept. of Medicinal Chemistry, the University of Utah

  • Richard Clark

    • BME Dept, SUNY Stony Brook
    • Dept. of Biomedical Engr., SUNY at Stony Brook

  • M. Rafailovich

    • SUNY
    • Dept. of Materials Science, SUNY at Stony Brook
    • Department of Materials Science, SUNY at Stony Brook
    • Dept. of Mat. Sci. and Engr,, State Univ. of New York at Stony Brook
    • Stony Brook Univ Stony Brook NY