Diffractive Imaging of Single Viruses

ORAL

Abstract

The resolution of Electron Microscopy (EM) images is limited by instrumentation lens aberration. Nanobeam electron diffraction in principle allows for diffraction-limited resolution analysis of single particles. However, images are not directly recoverable from the diffraction pattern due to the well-known phase problem. Algorithms for solving the phase problem have long been a topic of theoretical research, but only recently has diffractive imaging been experimentally actualized using coherent diffraction. We will discuss these algortihms and the potential of improving the resolution of TEM imaging of biomolecular systems through a combination of these algorithms and cryogenic sample preparation. Preliminary data on single virus diffraction data will be presented.

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Authors

  • Robert Coridan

    • Department of Physics, University of Illinois at Urbana-Champaign
    • Department of Physics, University of Illinois, Urbana-Champaign

  • John C. Butler

    • Department of Materials Science \& Engineering, University of Illinois at Urbana-Champaign
    • Department of Materials Science and Engineering

  • Thomas E. Angelini

    • Department of Physics, University of Illinois at Urbana-Champaign

  • Gerard C. L. Wong

    • Dept. of Materials Science and Engineering, Physics and Bioengineering, University of Illinois at Urbana-Champaign
    • Department of Materials Science \& Engineering, Department of Physics, Department of Bioengineering, University of Illinois at Urbana-Champaign
    • Department of Materials Science and Engineering, Department of Physics, Department of Bioengineering, University of Illinois at Urbana Champaign
    • University of Illinois at Urbana-Champaign
    • Department of Materials Science \& Engineering, Department of Physics, Department of Bioengineering, University of Illinois