Metabolomic profiles of glioblastoma in plasma-activated solutions

ORAL

Abstract

Cell culture media and Ringer's lactate solution irradiated by non-thermal plasma are defined as plasma-activated medium (PAM) and plasma-activated lactate (PAL), respectively. By analysis of metabolomic profiles of a hundred intracellular metabolites using capillary electrophoresis mass spectrometry, glioblastoma cells U251SP cultivated in PAM and PAL were changes in intracellular metabolites. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway. The metabolomic profiles of the PAL-treated U251SP were changed with generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells even in reductive microenvironments than PAM.

*This study was supported in part by Kakenhi Grants-in-Aid (Nos. 24108002 and 17H02850) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors

  • Kenji Ishikawa

    • Center for Low-temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601 Japan
    • Nagoya University, Nagoya, 464-8601, Japan
    • Nagoya University

  • Hiromasa Tanaka

    • Nagoya University

  • Li Jiang

    • Nagoya University

  • Shinya Toyokuni

    • Nagoya University

  • Kae Nakamura

    • Nagoya University

  • Hiroaki Kajiyama

    • Nagoya University

  • Fumitaka Kikkawa

    • Nagoya University

  • Masaaki Mizuno

    • Nagoya University

  • Camelia Miron

    • Nagoya University

  • Masaru Hori

    • Nagoya University