Influence of the H$_{2}$O$_{2}$ in the plasma gene transfection method

Gene transfection is the process of deliberately introducing nucleic acids into cells. The authors have been developing a novel gene transfection method using microplasma irradiation (plasma gene transfection method). Our previous study shows that long life chemically reactive species contribute to gene transfection, which induce the transfection at least 60~s after plasma irradiation (after effect). In order to clarify the key reactive species which is effective on the after effect, the effect of H$_{2}$O$_{2}$ addition after plasma irradiation was investigated. Addition of H$_{2}$O$_{2}$ at 1/1000$-$1~ppm after plasma irradiation did not largely affect or slightly decease the transfection ratio, whereas the H$_{2}$O$_{2}$ concentration induced by plasma irradiation is estimated as 2.7~ppb after dilution by the medium. It is found that the H$_{2}$O$_{2}$ is not main species for the after effect.