Optical magnetic detection of single-neuron action potentials using NV-diamond

ORAL

Abstract

A key challenge for neuroscience is noninvasive, label-free sensing of action potential dynamics in whole organisms with single-neuron resolution. Here, we report a new approach to this problem: using nitrogen-vacancy (NV) color centers in diamond to measure the time-dependent magnetic fields produced by single-neuron~action potentials. ~We demonstrate our method using excised single neurons from two invertebrate species, marine worm and squid; and then by single-neuron action potential magnetic sensing exterior to whole, live, opaque marine worms for extended periods with no adverse effect. The results lay the groundwork for real-time, noninvasive 3D magnetic mapping of functional mammalian neuronal networks.

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Authors

  • Matthew Turner

    • Harvard University
    • Department of Physics, Harvard University

  • John Barry

    • Harvard University
    • Harvard-Smithsonian Center for Astrophysics, Department of Physics, Harvard Unviersity

  • Jennifer Schloss

    • MIT

  • David Glenn

    • Harvard University

  • Ronald Walsworth

    • Harvard CfA
    • Harvard-Smithsonian Center for Astrophysics
    • Harvard-Smithsonian Center for Astrophysics and Harvard University
    • Harvard University
    • Harvard-Smithsonian Center for Astrophysics, Department of Physics, Harvard Unviersity