Techniques for super-resolution microscopy using NV-diamond

ORAL

Abstract

We discuss the development and application of techniques for super-resolution microscopy using NV centers in diamond: stimulated emission depletion (STED), metastable ground state depletion (GSD), and stochastic optical reconstruction microscopy (STORM). NV centers do not bleach under optical excitation, are not biotoxic, and have long-lived electronic spin coherence and spin-state-dependent fluorescence. Thus NV-diamond has great potential as a fluorescent biomarker and as a magnetic biosensor.

–

Authors

  • Alexei Trifonov

    • Harvard
    • Harvard University

  • David Glenn

    • Harvard-Smithsonian
    • Harvard-Smithsonian Center for Astrophysics
    • Harvard University

  • Nir Bar-Gill

    • Harvard-Smithsonian
    • Harvard-Smithsonian Center for Astrophysics
    • Harvard University

  • David Le Sage

    • Harvard-Smithsonian
    • Harvard University

  • Ronald Walsworth

    • Harvard-Smithsonian Center for Astrophysics; Department of Physics, Harvard University
    • Harvard-Smithsonian Center for Astrophysics
    • Harvard-Smithsonian
    • Harvard University